Exploring anticancer activity of the Indonesian guava leaf (<i>Psidium guajava</i> L.) fraction on various human cancer cell lines in an <i>in vitro</i> cell-based approach

Abstract Breast and cervical cancers are the leading cause of death in women, chemotherapy with cytotoxins is usual treatment. This study evaluated cytotoxicity guava leaf ( Psidium guajava L.) extracts as an alternative chemotherapeutic drug. Although many studies related to cytotoxic effects on cancer cells have been reported, fractions human breast (T47D, MCF-7, HeLa) never evaluated. Herein, we researched candidate activities ethanol, ethyl acetate, n -extracts from their effect various cell lines HeLa cells). The test was carried out using microtetrazolium assay for all fractions. We confirmed showed vitro antitumor activity cells. found that effectiveness anticancer increased ethanol acetate -hexane fraction. work underlines potential fraction a These novel results important implications further isolation, identification, characterization L. - based anti-cancer extracts.